why must the two arrows point toward one another to achieve successful PCR amplification?
this is an Genetic problem (about DNA replication)
why must the two arrows point toward one another to achieve successful PCR amplification?
The two stands of DNA are anti-parallel. Because DNA polymerase requires a primer, if you only primed in one direction, each new DNA molecule would lack the original priming site…the products would get shorter and shorter. If you have two priming sites coming from opposite ends, then the polymerase will blow right past the opposite priming site, making it long enough for the other primer to bind to that product and make a new produce (boy, that’s complicated to say!). Also remember that DNA polymerase can only add in the 5′ – 3′ direction, so having it shoot both ways (even if it COULD work in only one direction) would make the reaction only have as fast.
Turkish Archery: Bone arrow point making demo
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